Haematological and physiological responses of Piemontese beef cattle to different housing conditions

•Piemontese beef cattle are traditionally tie-stall housed during the fattening period.•We described the effect of tie-stall vs loose housing on physiological and haematological parameters.•The tie-stall housed cattle are more stressed than the loose housed animals.•The described alterations considered together may be effective markers of stress. Public concern for animal welfare has progressively grown over the recent years. In this context, stress has a great economical impact on growth of animals and quality of animal products. The development and validation of methods to assess animal stress, particularly at the farm level, are desirable to evaluate animal production systems. Piemontese breed is traditionally tie-stall housed in the fattening period. Hence, the objective of this study was to characterise a profile of physiological and haematological changes of Piemontese beef cattle under different management conditions (tie-stall and loose housing). Our results suggest that the housing system is an important factor in animal welfare. Indeed, the values of the total protein, lysozyme, cortisol, serum and faecal corticosterone concentration and GR-α gene expression indicate that the tie-stall housing is more stressful than the loose system. All the alterations highlighted in this study considered together may be effective biomarkers of stress and disease susceptibility.