A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O

A label-free fluorescent molecular switch for a DNA hybridization assay utilizing a G-quadruplex-selective auramine O

A sensitive and selective assay of DNA is developed by utilizing a signal transduction strategy with the rational redesign of the hairpin structured G-quadruplex molecular switch (G4-MS) assembled using auramine O (AO). By monitoring the changes of the fluorescent signal, we could identify and furth...

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Veröffentlicht in:Chemical communications (Cambridge, England) England), 2015-05, Vol.51 (41), p.8622-8625
Hauptverfasser: Xu, Huiying, Geng, Fenghua, Wang, Yongxiang, Xu, Maotian, Lai, Xinhe, Qu, Peng, Zhang, Yintang, Liu, Baohong
Format: Artikel
Sprache:eng
Schlagworte:
Assaying
Benzophenoneidum - chemistry
Deoxyribonucleic acid
DNA - analysis
Fluorescent Dyes - chemistry
G-Quadruplexes
Humans
Molecular Structure
Monitoring
Nucleic Acid Hybridization
Redesign
Strategy
Switches
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Beschreibung
Zusammenfassung:A sensitive and selective assay of DNA is developed by utilizing a signal transduction strategy with the rational redesign of the hairpin structured G-quadruplex molecular switch (G4-MS) assembled using auramine O (AO). By monitoring the changes of the fluorescent signal, we could identify and further quantitatively determine the target DNA in the samples.
ISSN:1359-7345
1364-548X
DOI:10.1039/c5cc02624e