Biofunction-assisted aptasensors based on ligand-dependent 3' processing of a suppressor tRNA in a wheat germ extract

We have developed a novel type of biofunction-assisted aptasensor that harnesses ligand-dependent 3' processing of a premature amber suppressor tRNA and the subsequent amber suppression of a reporter gene in a wheat germ extract.

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Bibliographische Detailangaben
Veröffentlicht in:	Organic & biomolecular chemistry 2015-06, Vol.13 (24), p.6681-6685
Hauptverfasser:	Ogawa, Atsushi, Tabuchi, Junichiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Aptamers, Nucleotide - chemistry Aptamers, Nucleotide - genetics Base Sequence Biosensing Techniques - methods Genes, Reporter Plant Extracts - chemistry Plant Extracts - genetics RNA, Transfer - chemistry RNA, Transfer - genetics Suppression, Genetic Theophylline - analysis Triticum - chemistry Triticum - genetics Vasodilator Agents - analysis
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Beschreibung
Zusammenfassung:	We have developed a novel type of biofunction-assisted aptasensor that harnesses ligand-dependent 3' processing of a premature amber suppressor tRNA and the subsequent amber suppression of a reporter gene in a wheat germ extract.
ISSN:	1477-0520 1477-0539
DOI:	10.1039/c5ob00794a