Rapid biorecognition assay for herbicides in biological matrices

A rapid assay capable of detecting several commonly used herbicides at nanogram per milliliter concentrations in biological fluids is described. The assay is based on inhibition of photosynthetic electron transport in spinach thylakoids by the target compounds with colorimetric detection using a red...

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Veröffentlicht in:	Analytical chemistry (Washington) 1993-09, Vol.65 (18), p.2415-2419
Hauptverfasser:	BREWSTER, J. D, LIGHTFIELD, A. R
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cattle Colorimetry Environmental monitoring Herbicides Herbicides - analysis Herbicides - blood Herbicides - urine Indicators and Reagents Meat - analysis Medical sciences Milk - chemistry Pesticide Residues - analysis Pesticide Residues - blood Pesticide Residues - urine Pesticides, fertilizers and other agrochemicals toxicology Plants - chemistry Toxicology
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Beschreibung
Zusammenfassung:	A rapid assay capable of detecting several commonly used herbicides at nanogram per milliliter concentrations in biological fluids is described. The assay is based on inhibition of photosynthetic electron transport in spinach thylakoids by the target compounds with colorimetric detection using a redox dye. Using a microtiter plate format, high throughput assays of water, urine, and homogenized tissue were performed in minutes with minimal sample preparation. Detection limits of 3 ng/mL for atrazine, 3 ng/mL for diuron, and 1 ng/mL for terbutryn were observed.
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac00066a004