A GENE ESSENTIAL FOR THE SITE-SPECIFIC EXCISION OF ACTINOPHAGE R4 PROPHAGE GENOME FROM THE CHROMOSOME OF A LYSOGEN

An open reading frame (ORF469) was found near the attP core site of the actinophage R4 which encoded a 469-amino acid polypeptide. A gene disruption experiment suggested that the ORF469 gene was essential for site-specific excision of the prophage R4 genome from its host chromosome. The predicted am...

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Veröffentlicht in:Journal of general and applied microbiology 1995, Vol.41(1), pp.53-61
Hauptverfasser: MATSUURA, MANABU, NOGUCHI, TAKAHIKO, AIDA, TOKUJIRO, ASAYAMA, MUNEHIKO, TAKAHASHI, HIDEO, SHIRAI, MAKOTO
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Sprache:eng
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Zusammenfassung:An open reading frame (ORF469) was found near the attP core site of the actinophage R4 which encoded a 469-amino acid polypeptide. A gene disruption experiment suggested that the ORF469 gene was essential for site-specific excision of the prophage R4 genome from its host chromosome. The predicted amino acid sequence of the protein showed a similarity to a resolvase of Tn2501 and SpoIVCA of Bacillus subtilis.
ISSN:0022-1260
1349-8037
DOI:10.2323/jgam.41.53