Major histocompatibility complex-restricted CD8 super(+) cytotoxic T lymphocytes from horses with equine infectious anemia virus recognize Env and Gag/PR proteins

Cytotoxic T lymphocytes (CTL) can control some viral infections and may be important in the control of lentiviruses, including human immunodeficiency virus type 1. Since there is limited evidence for an in vivo role of CTL in control of lentiviruses, dissection of immune mechanisms in animal lentiviral infections may provide needed information. Horses infected with equine infectious anemia virus (EIAV), a lentivirus, have acute plasma viremia which is terminated in immunocompetent horses. Viremic episodes may recur, but most horses ultimately control infection and become asymptomatic carriers. To begin dissection of the immune mechanisms involved in EIAV control, peripheral blood mononuclear cells (PBMC) from infected horses were evaluated for CTL to EIAV-infected cells. By using noninfected and EIAV-infected autologous equine kidney (EK) cells in super(51)Cr-release assays, EIAV-specific cytotoxic activity was detected in unstimulated PBMC from three infected horses. The detection of the immune effector cells in EIAV-infected horses permits further studies to determine their in vivo role.