Ieodoglucomide C and Ieodoglycolipid, New Glycolipids from a Marine-Derived Bacterium Bacillus licheniformis 09IDYM23

Chemical examination of the ethyl acetate extract from the fermentation broth of the marine-derived bacterium Bacillus licheniformis resulted in the isolation of two new glycolipids, ieodoglucomide C ( 1 ) and ieodoglycolipid ( 2 ). The structural characterization of 1 and 2 was achieved by extensive spectroscopic evidence, including 2D NMR experiments. A combination of chemical derivatization techniques followed by NMR studies, LC-MS data analysis and a literature review was deployed for the establishment of the stereo-configurations of 1 and 2 . Compounds 1 and 2 exhibited good antibiotic properties against Staphylococcus aureus , Bacillus subtilis , Bacillus cereus , Salmonella typhi , Escherichia coli and Pseudomonas aeruginosa with MICs ranging from 0.01 to 0.05 μM. Furthermore, the antifungal activity of 1 and 2 was evaluated against plant pathogenic fungi Aspergillus niger , Rhizoctonia solani , Botrytis cinerea and Colletotrichum acutatum as well as the human pathogen Candida albicans . Compounds 1 and 2 inhibited the mycelial growth of these pathogens with MIC values of 0.03–0.05 μM, revealing that these compounds are good candidates for the development of new fungicides.