Simultaneous determination of penicillins and sulfonamides in milk by high performance liquid chromatography

A simple and rapid method for the simultaneous determination of three penicillinase resistant penicillin derivatives (cloxacillin, dicloxacillin and nafcillin) and two sulfonamides (sulfamonomethoxine and sulfadimethoxine) in milk by high performance liquid chromatography (HPLC) was developed. The s...

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Veröffentlicht in:Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) 1993/10/05, Vol.34(5), pp.392-397_1
Hauptverfasser: Himei, R. (Kumamoto-ken. Government Office (Japan)), Koide, K, Tsuji, I, Yamamoto, S, Horie, M, Suzuki, S, Nakazawa, H
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Sprache:eng ; jpn
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Zusammenfassung:A simple and rapid method for the simultaneous determination of three penicillinase resistant penicillin derivatives (cloxacillin, dicloxacillin and nafcillin) and two sulfonamides (sulfamonomethoxine and sulfadimethoxine) in milk by high performance liquid chromatography (HPLC) was developed. The samples were prepared by extraction with acetonitrile followed by liquid-liquid partition with n-hexane for clean up. The extracts were analyzed by HPLC utilizing a UV detector with a timer which changed the wavelength of detection at a programmed time. The separation was achieved on TSK gel ODS-80TM (4.6×150mm i. d.) using acetonitrile: 0.05M NaH2PO4 (31:69) as a mobile phase. Recoveries of drugs from fortified milk at the levels of 0.1 and 0.5μg/ml were 77.3-82.1% and 88.2-91.7%, respectively. The detection limit in 4-step time program measurement was 0.02μg/ml for each drug.
ISSN:0015-6426
1882-1006
DOI:10.3358/shokueishi.34.392