THE GLUCOSE EFFECT ON LINCOMYCIN PRODUCTION BY STREPTOMYCES LINCOLNENSIS VAR. LINCOLNENSIS DMS 40 355 ON SYNTHETIC MEDIA

The objective of this study was to obtain the information about the lincomycin production by type strain Streptomyces lincolnensis var. lincolnensis DSM 40 355 on the synthetic medium. In order to improve the productivity, the ways of inoculation, effects of the medium components and fermentation time were investigated. The production of lincomycin was carried out at 30 °C on rotary shakers at 180 rpm and was tracked on 3rd, 7th, 10th, 12th, 14th, and 16th day of cultivation by using HPLC analysis. The results show that the highest production was observed on 16th day of cultivation on production M2 medium at 30 ± 1 ˚C, after inoculation with 1 ml of preculture M1 medium, and after an additional supplementation of the media with 0.5 g of glucose per 50 ml medium on 7th day of cultivation. Preculture medium application and glucose addition had positive effect, resulting in a remarkable increase in the lincomycin production.