A serine (threonine) protein kinase confers fungicide resistance in the phytopathogenic fungus Ustilago maydis

A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance-conferring fragment was isolated by sib selection. Sequencing this fragment, we identified...

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Veröffentlicht in:	Applied and Environmental Microbiology 1995-06, Vol.61 (6), p.2341-2345
Hauptverfasser:	Orth, A.B. (DowElanco Discovery Research, Indianapolis, IN.), Rzhetskaya, M, Pell, E.J, Tien, M
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences COMPOSICION QUIMICA COMPOSITION CHIMIQUE DNA-Binding Proteins - genetics Drug Resistance, Microbial FONGICIDE Fundamental and applied biological sciences. Psychology Fungal plant pathogens Fungi FUNGICIDAS GENE Gene Deletion GENES Molecular Sequence Data Oxazoles - pharmacology Phytopathology. Animal pests. Plant and forest protection Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism PROTEINA QUINASA PROTEINE KINASE Proteins RESISTANCE AUX PRODUITS CHIMIQUES RESISTENCIA A PRODUCTOS QUIMICOS Saccharomyces cerevisiae Proteins SECUENCIA NUCLEICA Sequence Alignment SEQUENCE NUCLEIQUE Systematics. Morphology. Development cycle. Physiology Transcription Factors - genetics USTILAGO Ustilago - enzymology Ustilago - genetics Ustilago maydis VINCHLOZOLINE VINCLOZOLINA
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Zusammenfassung:	A mutant of Ustilago maydis (VR43) with single-gene resistance to the dicarboximide fungicide vinclozolin was previously isolated and characterized. A genomic library was constructed, and an 8.7-kb resistance-conferring fragment was isolated by sib selection. Sequencing this fragment, we identified an 1,218-bp open reading frame, which, if disrupted by deletion, no longer confers resistance. Analyses of the data in GenBank demonstrated a high degree of homology between the product of the 1,218-bp open reading frame, referred to as the adr-1 gene, and Ser (Thr) protein kinases
ISSN:	0099-2240 1098-5336
DOI:	10.1128/aem.61.6.2341-2345.1995