Aquisalimonas lutea sp. nov., a moderately halophilic bacterium from a saltern

A yellow-pigmented, motile, Gram-stain-negative, moderately halophilic and strictly aerobic bacterium, designated BA42AL-1 ᵀ, was isolated from water of a saltern of Santa Pola, Alicante, Spain. Strain BA42AL-1 ᵀ grew in media containing 5–20 % (w/v) salts (optimum 7.5 % salts). It grew between pH 6.0 and 9.0 (optimally at pH 7.5) and at 15–45 °C (optimally at 37 °C). Phylogenetic analysis based on the comparison of 16S rRNA gene sequences revealed that strain BA42AL-1 ᵀ is a member of the genus Aquisalimonas . The closest relatives to this strain were Aquisalimonas halophila YIM 95345 ᵀ and Aquisalimonas asiatica CG12 ᵀ with sequence similarities of 99.4 % and 97.0 %, respectively. DNA–DNA hybridization between the novel isolate and Aquisalimonas halophila YIM 95345 ᵀ revealed a relatedness of 54 %. The major fatty acids of strain BA42AL-1 ᵀ were C ₁₈ : ₁ω6 c /C ₁₈ : ₁ω7 c , C ₁₉ : ₀ cyclo ω 8c and C ₁₆ : ₀, and lower contents of C ₁₂ : ₀ and C ₁₈ : ₀. The polar lipid pattern of strain BA42AL-1 ᵀ consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylcholine, two glycolipids, a lipid and four unknown phospholipids. The G+C content of the genomic DNA of this strain was 65.0 mol%. Based on the DNA–DNA hybridization, phenotypic, chemotaxonomic and phylogenetic data presented in this study, strain BA42AL-1 ᵀ is proposed as a novel species of the genus Aquisalimonas , for which the name Aquisalimonas lutea sp. nov. is suggested. The type strain is BA42AL-1 ᵀ (= CCM 8472 ᵀ = CECT 8326 ᵀ = LMG 27614 ᵀ).