Production of the lepidoptera-specific crystal protein by a local isolate of Bacillus thuringiensis

A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein which was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; pr...

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Veröffentlicht in:Biotechnology letters 1993-12, Vol.15 (12), p.1253-1258
Hauptverfasser: AFKHAMI, P, ÖZCENGIZ, G, ALAEDDINOGLU, N. G
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Sprache:eng
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Zusammenfassung:A local isolate of Bacillus thuringiensis (B.t.81) produced crystal protein which was identified as a cry I gene of Class I. The synthesis and assembly of crystal complements were investigated at intervals throughout the growth cycle. Incubation temperature had a marked effect on toxin synthesis; production being the highest at 25 degree C and the lowest at 42 degree C. The mutants of B. t. 81, unable to synthesize crystal protein complements, have also been described.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00130307