Modulation of functional activities of the neurotoxin from black widow spider venom

We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [ 14C[GABA release from s...

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Veröffentlicht in:	FEBS letters 1993-12, Vol.336 (2), p.205-207
Hauptverfasser:	Grishin, E.V., Himmelreich, N.H., Pluzhnikov, K.A., Pozdnyakova, N.G., Storchak, L.G., Volkova, T.M., Woll, P.G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Araneae Black Widow Spider - chemistry Calcium influx GABA release gamma-Aminobutyric Acid - metabolism Molecular Sequence Data Molecular Weight PC12 cell PC12 Cells Proteins - chemistry Proteins - pharmacology Rat brain synaptosome Rats Spider Venoms - chemistry Spider Venoms - pharmacology Synaptosomes - drug effects Synaptosomes - metabolism Theridiidae α-Latrotoxin
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Zusammenfassung:	We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [ 14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [ 14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(93)80803-3