Arthropod predation: a simplified immunodot format for predator gut analysis

We describe a new immunodot format which retains the speed, sensitivity, economy, and specificity of the previously published assay but eliminates some apparatus and is simpler to perform. Assays are run on nitrocellulose membranes onto which predator homogenates are applied directly; they use monoc...

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Veröffentlicht in:	Annals of the Entomological Society of America 1994-03, Vol.87 (2), p.214-217
Hauptverfasser:	Greenstone, M.H, Trowell, S.C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal and plant ecology Animal, plant and microbial ecology Animals ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES APPAREIL DIGESTIF Autoecology Biological and medical sciences DEPREDADORES DIGESTIVE SYSTEM DIGESTIVE TRACT Fundamental and applied biological sciences. Psychology HELICOVERPA ZEA IMMUNOASSAY IMMUNOLOGICAL TECHNIQUES Insecta Invertebrates MONOCLONAL ANTIBODIES PODISUS MACULIVENTRIS PREDATEUR PREDATORS Protozoa. Invertebrata SISTEMA DIGESTIVO TECHNIQUE IMMUNOLOGIQUE TECNICAS INMUNOLOGICAS
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Beschreibung
Zusammenfassung:	We describe a new immunodot format which retains the speed, sensitivity, economy, and specificity of the previously published assay but eliminates some apparatus and is simpler to perform. Assays are run on nitrocellulose membranes onto which predator homogenates are applied directly; they use monoclonal antibodies to prey egg or larval proteins, and can be interpreted by eye. The assay detects the remains of a single egg or fifth instar of Helicoverpa zea (Boddie) in the gut of a second-instar or adult Podisus maculiventris (Say), respectively.
ISSN:	0013-8746 1938-2901
DOI:	10.1093/aesa/87.2.214