On plasma volume measurement and the effect of experimental stress in the male tilapia, Oreochromis mossambicus, maintained in fresh water

Plasma volumes in male tilapia (Oreochromis mossambicus) of different size were estimated following intracardial injection of radioiodinated human serum albumin ( super(125)I-HSA), coupled with short-term, early sampling transient response analysis of super(125)I-HSA disappearance from the plasma pool. This approach circumvents vascular marker leakage problems associated with constant steady state indicator dilution methods, minimizes some sampling and mixing problems, and simplifies analysis of the data.