Effect of reaction conditions on L-histidine decarboxylation activity of halophilic histamine-forming bacteria

Effect of reaction conditions on L-histidine decarboxylation activity of halophilic histamine-forming bacteria

the effect of reaction conditions on L-histidine decarboxylation activity of halophilic histamine-forming bacteria (Photobacterium histaminum (C-8) and P. phosphoreum (N-14)) were studied and the results were as follows. The opitimum temperature and pH value for the decarboxylation activity of C-8 w...

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Veröffentlicht in:NIPPON SUISAN GAKKAISHI 1993/10/25, Vol.59(10), pp.1745-1748
Hauptverfasser: Kurihara, K. (Tokyo Univ. of Fisheries (Japan)), Wagatsuma, Y, Fujii, T, Okuzumi, M
Format: Artikel
Sprache:eng ; jpn
Schlagworte:
BACTERIA
CARBONATE
CARBONATES
CARBONATOS
CHEMICAL REACTIONS
HALOFITAS
HALOPHYTE
HALOPHYTES
HISTAMINA
HISTAMINE
HISTIDINA
HISTIDINE
Marine
PH
Photobacterium
REACCIONES QUIMICAS
REACTION CHIMIQUE
TEMPERATURA
TEMPERATURE
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Zusammenfassung:the effect of reaction conditions on L-histidine decarboxylation activity of halophilic histamine-forming bacteria (Photobacterium histaminum (C-8) and P. phosphoreum (N-14)) were studied and the results were as follows. The opitimum temperature and pH value for the decarboxylation activity of C-8 were 35°C and 6.0, respectively, while those for N-14 were 30 ?? 35°C and 6.0, respectively. The Km value for C-8 (3.70mM) was 5 times smaller than that for N-14 (18.8mM), showing a powerful affinity for histidine. The presence of histamine showed an inhibitory effect for the histidine decarboxylation activity of C-8, while agmatine and putrescine besides histamine did so for N-14.
ISSN:0021-5392
1349-998X
DOI:10.2331/suisan.59.1745