A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom

A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom

A highly sensitive fluorogenic enzyme‐linked immunosorbent assay (FELISA) has been adapted for the detection of Vipera russelli venom. The assay sensitivity was observed to be 0.1 pg/ml (1 × 10−13 g/ml). Venoms from snakes of the Vipera group exhibited a high degree of cross‐reactivity when tested w...

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Veröffentlicht in:Natural toxins 1993, Vol.1 (5), p.277-282
Hauptverfasser: Bhatti, Abdul R., Wong, Jonathan P., Siddiqui, Yunus M., Siddiqui, Shahnaz
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Cross Reactions
ELISA
Enzyme-Linked Immunosorbent Assay
Male
Mice
Mice, Inbred BALB C
Sensitivity and Specificity
Snake venom
Viper Venoms - analysis
Viper Venoms - immunology
Viper Venoms - toxicity
Vipera russelli
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container_end_page 282
container_issue 5
container_start_page 277
container_title Natural toxins
container_volume 1
creator Bhatti, Abdul R.
Wong, Jonathan P.
Siddiqui, Yunus M.
Siddiqui, Shahnaz
description A highly sensitive fluorogenic enzyme‐linked immunosorbent assay (FELISA) has been adapted for the detection of Vipera russelli venom. The assay sensitivity was observed to be 0.1 pg/ml (1 × 10−13 g/ml). Venoms from snakes of the Vipera group exhibited a high degree of cross‐reactivity when tested with the antibody raised against V. russelli venom. With the exception of venom from Naja naja, all the tested venoms from unrelated families also showed cross‐reactivity. This procedure is useful for detecting snake venom or its components in biological samples. © 1992 Wiley‐Liss, Inc.
doi_str_mv 10.1002/nt.2620010504
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Animals
Cross Reactions
ELISA
Enzyme-Linked Immunosorbent Assay
Male
Mice
Mice, Inbred BALB C
Sensitivity and Specificity
Snake venom
Viper Venoms - analysis
Viper Venoms - immunology
Viper Venoms - toxicity
Vipera russelli
title A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom
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