A sensitive fluorogenic enzyme linked immunosorbent assay for the detection of Vipera russelli venom

A highly sensitive fluorogenic enzyme‐linked immunosorbent assay (FELISA) has been adapted for the detection of Vipera russelli venom. The assay sensitivity was observed to be 0.1 pg/ml (1 × 10−13 g/ml). Venoms from snakes of the Vipera group exhibited a high degree of cross‐reactivity when tested with the antibody raised against V. russelli venom. With the exception of venom from Naja naja, all the tested venoms from unrelated families also showed cross‐reactivity. This procedure is useful for detecting snake venom or its components in biological samples. © 1992 Wiley‐Liss, Inc.