Super-resolution imaging with stochastic single-molecule localization: Concepts, technical developments, and biological applications

Super-resolution imaging with stochastic single-molecule localization: Concepts, technical developments, and biological applications

ABSTRACT Light microscopy has undergone a revolution with the advent of super‐resolution microscopy methods that can surpass the diffraction limit. These methods have generated much enthusiasm, in particular with regards to the new possibilities they offer for biological imaging. The recent years ha...

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Veröffentlicht in:Microscopy research and technique 2014-07, Vol.77 (7), p.502-509
Hauptverfasser: Oddone, Anna, Vilanova, Ione Verdeny, Tam, Johnny, Lakadamyali, Melike
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biological
Cells - ultrastructure
Diffraction
Excitation
fluorescence microscopy
Fluorescent Dyes
Humans
Image Interpretation, Computer-Assisted - methods
Imaging
Imaging, Three-Dimensional - methods
Localization
Microscopy
Microscopy, Fluorescence - methods
PALM
Position (location)
Stochastic Processes
Stochasticity
STORM
super resolution
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Zusammenfassung:ABSTRACT Light microscopy has undergone a revolution with the advent of super‐resolution microscopy methods that can surpass the diffraction limit. These methods have generated much enthusiasm, in particular with regards to the new possibilities they offer for biological imaging. The recent years have seen a great advancement both in terms of new technological developments and exciting biological applications. Here, we review some of the important milestones in the field and highlight some recent biological applications. Microsc. Res. Tech. 77:502–509, 2014. © 2014 Wiley Periodicals, Inc.
ISSN:1059-910X
1097-0029
DOI:10.1002/jemt.22346