Preparation of temperature sensitive molecularly imprinted polymer for solid-phase microextraction coatings on stainless steel fiber to measure ofloxacin

[Display omitted] •Dopamine was modified on stainless steel fiber as MIP fiber support.•The stainless steel fiber was put into a capillary for the synthesis.•Several capillaries were put together in same synthesis solution.•The obtained MIP was evaluated well using different techniques.•The MIP fiber was used to pretreat the milk samples to measure ofloxacin. A kind of new temperature sensitive molecularly imprinted polymer (MIP) with ofloxacin (OFL) as template was prepared for the coating of solid phase microextraction (SPME). Dopamine was self-polymerized on stainless steel fiber (SSF) as the SPME support followed by silanization. Then MIP was synthesized as SPME coating on the modified SSF in a capillary, with N-isopropyl acrylamide as temperature sensitive monomer and methacrylic acid as functional monomer. The synthesis could be well repeated with multiple capillaries putting in the same reaction solution. The obtained MIP fiber was evaluated in detail with different techniques and various adsorption experiments. At last the MIP fiber was used to extract the OFL in milk. Satisfied recoveries between 89.7 and 103.4% were obtained with the limit of quantification (LOQLC) of 0.04μgmL−1 by the method of SPME coupled with high performance of liquid chromatography (HPLC).