Identification of C-type isolectins in the venom of the scorpionfish Scorpaena plumieri

Chemical analyses of the hemagglutinating fraction from Scorpaena plumieri venom revealed that it contains five components (Sp-CL 1-5) with similar chromatographic elution profiles (35–38% of acetonitrile), molecular masses (16,800–17,000 Da) and N-terminal sequences, suggesting that they are isoforms of the same protein. The amino acid sequence of Sp-CL4 was determined and shown to have homology with fish C-type lectins. These data demonstrate for the first time the presence of C-type isolectins in a scorpionfish venom. •A hemagglutinating fraction (FV) was purified from Scorpaena plumieri fish venom.•FV contains five components with similar molecular masses and N-terminal sequences.•These compounds were purified and named Sp-CL 1-5.•Sp-CL4 amino acid sequence exhibited homology with galactose-binding C-type lectins.•We demonstrate the presence of C-type lectins isoforms in scorpionfish venom.