Improved stability and efficacy of chitosan/pDNA complexes for gene delivery

Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charg...

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Veröffentlicht in:Biotechnology letters 2015-03, Vol.37 (3), p.557-565
Hauptverfasser: Cifani, Noemi, Chronopoulou, Laura, Pompili, Barbara, Di Martino, Antonio, Bordi, Federico, Sennato, Simona, Di Domenico, Enea Gino, Palocci, Cleofe, Ascenzioni, Fiorentina
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Sprache:eng
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Zusammenfassung:Among polymeric polycations, chitosan has emerged as a powerful carrier for gene delivery. Only a few studies have focused on the stability of the chitosan/DNA complex under storage, although this is imperative for nanomedicinal applications. Here, we synthesized polyelectrolyte complexes at a charge ratio of 10 using 50 kDa chitosan and plasmid (p)DNA that encodes a GFP reporter. These preparations were stable up to 3 months at 4 °C and showed reproducible transfection efficiencies in vitro in HEK293 cells. In addition, we developed a methodology that increases the in vitro transfection efficiency of chitosan/pDNA complexes by 150 % with respect to standard procedures. Notably, intracellular pDNA release and transfected cells peaked 5 days following transection of mitotically active cells. These new developments in formulation technology enhance the potential for polymeric nanoparticle-mediated gene therapy.
ISSN:0141-5492
1573-6776
DOI:10.1007/s10529-014-1727-7