Transfection of Mammalian Cells Using Block Copolypeptide Vesicles
An arginine‐leucine block copolypeptide (R60L20) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60L20 vesicles also possess the ability to deliver DNA into mamma...
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| Veröffentlicht in: | Macromolecular bioscience 2013-05, Vol.13 (5), p.539-550 |
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| creator | Sun, Victor Z. Choe, Uh-Joo Rodriguez, April R. Dai, Howard Deming, Timothy J. Kamei, Daniel T. |
| description | An arginine‐leucine block copolypeptide (R60L20) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60L20 vesicles also possess the ability to deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, the R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. This behavior is potentially due to its stronger interaction with DNA which subsequently provides better protection against anionic heparin.
Poly(L‐arginine)60‐block‐poly(L‐leucine)20 (R60L20) vesicles possess the ability to internalize cells and deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. |
| doi_str_mv | 10.1002/mabi.201200383 |
| format | Article |
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Poly(L‐arginine)60‐block‐poly(L‐leucine)20 (R60L20) vesicles possess the ability to internalize cells and deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity.</description><identifier>ISSN: 1616-5187</identifier><identifier>EISSN: 1616-5195</identifier><identifier>DOI: 10.1002/mabi.201200383</identifier><identifier>PMID: 23460310</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Animals ; Anions ; block copolymers ; Blocking ; Deoxyribonuclease I - metabolism ; Deoxyribonucleic acid ; DNA - metabolism ; Enzyme-Linked Immunosorbent Assay ; Flow Cytometry ; Forming ; HeLa Cells ; Heparins ; Humans ; immunogenicity ; Interleukin-6 - metabolism ; Light ; Lipids ; Luminescent Proteins - metabolism ; Membranes ; Mice ; Microscopy, Confocal ; peptides ; Peptides - pharmacology ; Plasmids - metabolism ; Scattering, Radiation ; Stability ; transfection ; Transfection - methods ; Transport ; Unilamellar Liposomes - chemistry ; Vesicles</subject><ispartof>Macromolecular bioscience, 2013-05, Vol.13 (5), p.539-550</ispartof><rights>Copyright © 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4163-3b123dc1fd7f15ea866a717a6f9e2acb3539ca952a042d8edb61ea651968073a3</citedby><cites>FETCH-LOGICAL-c4163-3b123dc1fd7f15ea866a717a6f9e2acb3539ca952a042d8edb61ea651968073a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fmabi.201200383$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fmabi.201200383$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23460310$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sun, Victor Z.</creatorcontrib><creatorcontrib>Choe, Uh-Joo</creatorcontrib><creatorcontrib>Rodriguez, April R.</creatorcontrib><creatorcontrib>Dai, Howard</creatorcontrib><creatorcontrib>Deming, Timothy J.</creatorcontrib><creatorcontrib>Kamei, Daniel T.</creatorcontrib><title>Transfection of Mammalian Cells Using Block Copolypeptide Vesicles</title><title>Macromolecular bioscience</title><addtitle>Macromol. Biosci</addtitle><description>An arginine‐leucine block copolypeptide (R60L20) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60L20 vesicles also possess the ability to deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, the R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. This behavior is potentially due to its stronger interaction with DNA which subsequently provides better protection against anionic heparin.
Poly(L‐arginine)60‐block‐poly(L‐leucine)20 (R60L20) vesicles possess the ability to internalize cells and deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity.</description><subject>Animals</subject><subject>Anions</subject><subject>block copolymers</subject><subject>Blocking</subject><subject>Deoxyribonuclease I - metabolism</subject><subject>Deoxyribonucleic acid</subject><subject>DNA - metabolism</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Flow Cytometry</subject><subject>Forming</subject><subject>HeLa Cells</subject><subject>Heparins</subject><subject>Humans</subject><subject>immunogenicity</subject><subject>Interleukin-6 - metabolism</subject><subject>Light</subject><subject>Lipids</subject><subject>Luminescent Proteins - metabolism</subject><subject>Membranes</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>peptides</subject><subject>Peptides - pharmacology</subject><subject>Plasmids - metabolism</subject><subject>Scattering, Radiation</subject><subject>Stability</subject><subject>transfection</subject><subject>Transfection - methods</subject><subject>Transport</subject><subject>Unilamellar Liposomes - chemistry</subject><subject>Vesicles</subject><issn>1616-5187</issn><issn>1616-5195</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EonytjCgjS4rPTuxkpBUUpBaWUqQu1sW5IEO-iFtB_z1BhYqNyTc873O-l7Fz4EPgXFxVmLmh4CA4l4ncY0egQIUxpPH-bk70gB17_8o56CQVh2wgZKS4BH7ERvMOa1-QXbmmDpoimGFVYemwDsZUlj548q5-CUZlY9-CcdM25aalduVyChbknS3Jn7KDAktPZz_vCXu6vZmP78Lp4-R-fD0NbQRKhjIDIXMLRa4LiAkTpVCDRlWkJNBmMpapxTQWyCORJ5RnCghVf4pKuJYoT9jl1tt2zfua_MpUztv-k1hTs_YGlNZpJJOE_4_2y3gUpUr16HCL2q7xvqPCtJ2rsNsY4Oa7YvNdsdlV3AcuftzrrKJ8h_922gPpFvhwJW3-0ZnZ9ej-rzzcZp1f0ecui92bUVrq2Dw_TMwymi9gqWZGyy88PpXh</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>Sun, Victor Z.</creator><creator>Choe, Uh-Joo</creator><creator>Rodriguez, April R.</creator><creator>Dai, Howard</creator><creator>Deming, Timothy J.</creator><creator>Kamei, Daniel T.</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7SR</scope><scope>7U5</scope><scope>8FD</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>201305</creationdate><title>Transfection of Mammalian Cells Using Block Copolypeptide Vesicles</title><author>Sun, Victor Z. ; Choe, Uh-Joo ; Rodriguez, April R. ; Dai, Howard ; Deming, Timothy J. ; Kamei, Daniel T.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4163-3b123dc1fd7f15ea866a717a6f9e2acb3539ca952a042d8edb61ea651968073a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Animals</topic><topic>Anions</topic><topic>block copolymers</topic><topic>Blocking</topic><topic>Deoxyribonuclease I - metabolism</topic><topic>Deoxyribonucleic acid</topic><topic>DNA - metabolism</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Flow Cytometry</topic><topic>Forming</topic><topic>HeLa Cells</topic><topic>Heparins</topic><topic>Humans</topic><topic>immunogenicity</topic><topic>Interleukin-6 - metabolism</topic><topic>Light</topic><topic>Lipids</topic><topic>Luminescent Proteins - metabolism</topic><topic>Membranes</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>peptides</topic><topic>Peptides - pharmacology</topic><topic>Plasmids - metabolism</topic><topic>Scattering, Radiation</topic><topic>Stability</topic><topic>transfection</topic><topic>Transfection - methods</topic><topic>Transport</topic><topic>Unilamellar Liposomes - chemistry</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sun, Victor Z.</creatorcontrib><creatorcontrib>Choe, Uh-Joo</creatorcontrib><creatorcontrib>Rodriguez, April R.</creatorcontrib><creatorcontrib>Dai, Howard</creatorcontrib><creatorcontrib>Deming, Timothy J.</creatorcontrib><creatorcontrib>Kamei, Daniel T.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Macromolecular bioscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sun, Victor Z.</au><au>Choe, Uh-Joo</au><au>Rodriguez, April R.</au><au>Dai, Howard</au><au>Deming, Timothy J.</au><au>Kamei, Daniel T.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfection of Mammalian Cells Using Block Copolypeptide Vesicles</atitle><jtitle>Macromolecular bioscience</jtitle><addtitle>Macromol. Biosci</addtitle><date>2013-05</date><risdate>2013</risdate><volume>13</volume><issue>5</issue><spage>539</spage><epage>550</epage><pages>539-550</pages><issn>1616-5187</issn><eissn>1616-5195</eissn><abstract>An arginine‐leucine block copolypeptide (R60L20) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60L20 vesicles also possess the ability to deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, the R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. This behavior is potentially due to its stronger interaction with DNA which subsequently provides better protection against anionic heparin.
Poly(L‐arginine)60‐block‐poly(L‐leucine)20 (R60L20) vesicles possess the ability to internalize cells and deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>23460310</pmid><doi>10.1002/mabi.201200383</doi><tpages>12</tpages></addata></record> |
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| subjects | Animals Anions block copolymers Blocking Deoxyribonuclease I - metabolism Deoxyribonucleic acid DNA - metabolism Enzyme-Linked Immunosorbent Assay Flow Cytometry Forming HeLa Cells Heparins Humans immunogenicity Interleukin-6 - metabolism Light Lipids Luminescent Proteins - metabolism Membranes Mice Microscopy, Confocal peptides Peptides - pharmacology Plasmids - metabolism Scattering, Radiation Stability transfection Transfection - methods Transport Unilamellar Liposomes - chemistry Vesicles |
| title | Transfection of Mammalian Cells Using Block Copolypeptide Vesicles |
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