Transfection of Mammalian Cells Using Block Copolypeptide Vesicles

An arginine‐leucine block copolypeptide (R60L20) is synthesized, which is capable of forming vesicles with controllable sizes, able to transport hydrophilic cargo across the cell membrane, and exhibit relatively low cytotoxicity. The R60L20 vesicles also possess the ability to deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, the R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity. This behavior is potentially due to its stronger interaction with DNA which subsequently provides better protection against anionic heparin. Poly(L‐arginine)60‐block‐poly(L‐leucine)20 (R60L20) vesicles possess the ability to internalize cells and deliver DNA into mammalian cells for transfection. Although the transfection efficiency is lower than that of the commercially available transfection agent Lipofectamine 2000, R60L20 vesicles are able to achieve transfection with significantly lower cytotoxicity and immunogenicity.