Generation of Cellular Micropatterns on a Single-Layered Graphene Film

Micropatterns of fibroblast and hippocampal neurons are generated on a single‐layered graphene substrate. A large‐area (1 cm × 1 cm) graphene film on Si/SiO2 is functionalized by surface‐initiated ATRP of non‐biofouling oligo(ethylene glycol) methacrylate, after grafting of the polymerization initiator bearing α‐bromoisobutylate via photoreaction of the perfluorophenyl azide group. The microcontact printing‐assisted spatio‐selective reaction, after chemical activation of the terminal hydroxyl group of oligo(ethylene glycol) in the polymeric film, is utilized to generate the patterns of fibroblast and hippocampal neurons. Micropatterns of NIH 3T3 fibroblast cells or hippocampal neurons are generated on a single‐layered graphene film by a combination of SI‐ATRP and microcontact printing after photoreaction‐based grafting of the SI‐ATRP initiator.