Effects of laccase-catalyzed rutin polymer fraction on adipogenesis inhibition in 3T3-L1 adipocytes
•Laccase-catalyzed oxidation of rutin created novel biopolymers.•Rutin polymer fraction (RPF, hexamer to octamer) effectively suppressed triglyceride accumulation compared to the rutin monomer.•The anti-adipogenic effect of RPF was confirmed in the expression of adipogenic transcriptional factors. R...
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| Veröffentlicht in: | Process biochemistry (1991) 2014-07, Vol.49 (7), p.1189-1195 |
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| container_title | Process biochemistry (1991) |
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| creator | Jeon, Ji Kyeong Lee, Jihye Imm, Jee-Young |
| description | •Laccase-catalyzed oxidation of rutin created novel biopolymers.•Rutin polymer fraction (RPF, hexamer to octamer) effectively suppressed triglyceride accumulation compared to the rutin monomer.•The anti-adipogenic effect of RPF was confirmed in the expression of adipogenic transcriptional factors.
Rutin was enzymatically polymerized by laccase and the rutin polymer fraction (RPF) was collected by ultrafiltration with a molecular cut off of 3kDa. The RPF contained a range of oligomer size varying from hexamer (m/z 3650) to octamer (m/z 4866). The Fourier transform infrared spectrum indicated that various linkages such as hydrogen bonds (OH), aromatic groups (CC), and ether bonds (CO) were involved in rutin polymer formation. RPF more effectively suppressed triglyceride accumulation compared to the rutin monomer in 3T3-L1 adipocytes at all tested concentrations (p |
| doi_str_mv | 10.1016/j.procbio.2014.04.005 |
| format | Article |
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Rutin was enzymatically polymerized by laccase and the rutin polymer fraction (RPF) was collected by ultrafiltration with a molecular cut off of 3kDa. The RPF contained a range of oligomer size varying from hexamer (m/z 3650) to octamer (m/z 4866). The Fourier transform infrared spectrum indicated that various linkages such as hydrogen bonds (OH), aromatic groups (CC), and ether bonds (CO) were involved in rutin polymer formation. RPF more effectively suppressed triglyceride accumulation compared to the rutin monomer in 3T3-L1 adipocytes at all tested concentrations (p<0.05). The anti-adipogenic effect of RPF was mediated by down-regulation of the expression of adipogenic transcriptional factors including peroxisome proliferator-activated receptor (PPARγ), CCATT/enhancer binding protein (C/EBPα), sterol regulatory binding protein (SREBP1C) and fatty acid synthase (FAS) while up-regulating lipolysis and adiponectin expression in 3T3-L1 adipocytes. These results suggest that RPF has a potential protective effect in the development of obesity and that enzyme-catalyzed polymerization could be useful in improving flavonoids bioactivity.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2014.04.005</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>3T3-L1 cells ; Anti-adipogenic activity ; Binding ; Biochemistry ; Ethers ; Fatty acids ; Laccase ; Monomers ; Oligomers ; Polymerization ; Proteins ; Rutin</subject><ispartof>Process biochemistry (1991), 2014-07, Vol.49 (7), p.1189-1195</ispartof><rights>2014 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-816ecc333b8270b6c4029c0730e797b7340b4fb7de95e0fa8b824afc92f8b4293</citedby><cites>FETCH-LOGICAL-c478t-816ecc333b8270b6c4029c0730e797b7340b4fb7de95e0fa8b824afc92f8b4293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1359511314002293$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids></links><search><creatorcontrib>Jeon, Ji Kyeong</creatorcontrib><creatorcontrib>Lee, Jihye</creatorcontrib><creatorcontrib>Imm, Jee-Young</creatorcontrib><title>Effects of laccase-catalyzed rutin polymer fraction on adipogenesis inhibition in 3T3-L1 adipocytes</title><title>Process biochemistry (1991)</title><description>•Laccase-catalyzed oxidation of rutin created novel biopolymers.•Rutin polymer fraction (RPF, hexamer to octamer) effectively suppressed triglyceride accumulation compared to the rutin monomer.•The anti-adipogenic effect of RPF was confirmed in the expression of adipogenic transcriptional factors.
Rutin was enzymatically polymerized by laccase and the rutin polymer fraction (RPF) was collected by ultrafiltration with a molecular cut off of 3kDa. The RPF contained a range of oligomer size varying from hexamer (m/z 3650) to octamer (m/z 4866). The Fourier transform infrared spectrum indicated that various linkages such as hydrogen bonds (OH), aromatic groups (CC), and ether bonds (CO) were involved in rutin polymer formation. RPF more effectively suppressed triglyceride accumulation compared to the rutin monomer in 3T3-L1 adipocytes at all tested concentrations (p<0.05). The anti-adipogenic effect of RPF was mediated by down-regulation of the expression of adipogenic transcriptional factors including peroxisome proliferator-activated receptor (PPARγ), CCATT/enhancer binding protein (C/EBPα), sterol regulatory binding protein (SREBP1C) and fatty acid synthase (FAS) while up-regulating lipolysis and adiponectin expression in 3T3-L1 adipocytes. These results suggest that RPF has a potential protective effect in the development of obesity and that enzyme-catalyzed polymerization could be useful in improving flavonoids bioactivity.</description><subject>3T3-L1 cells</subject><subject>Anti-adipogenic activity</subject><subject>Binding</subject><subject>Biochemistry</subject><subject>Ethers</subject><subject>Fatty acids</subject><subject>Laccase</subject><subject>Monomers</subject><subject>Oligomers</subject><subject>Polymerization</subject><subject>Proteins</subject><subject>Rutin</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqFkF1LwzAUhosoOKc_QeilN60nTds0VyJjfsDAm3kd0tMTzeiamnTC_PVmzvvBC-dAnvNCniS5ZZAzYPX9Jh-9w9a6vABW5hAD1VkyY43gGS9kcx53XsmsYoxfJlchbAA4YwxmCS6NIZxC6kzaa0QdKEM96X7_Q13qd5Md0tH1-y351HiNk3VDGqM7O7oPGijYkNrh07b27ynifM2zFTsSuJ8oXCcXRveBbv7nPHl_Wq4XL9nq7fl18bjKsBTNlDWsJkTOedsUAtoaSygkguBAQopW8BLa0rSiI1kRGN1ErtQGZWGatiwknyd3x96o42tHYVJbG5D6Xg_kdkGxWghZ8KooT6NVDYxzJg-t1RFF70LwZNTo7Vb7vWKgDv7VRv37Vwf_CmKgincPxzuKX_625FVASwNSZ300rjpnTzT8Aj_IkVQ</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Jeon, Ji Kyeong</creator><creator>Lee, Jihye</creator><creator>Imm, Jee-Young</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7U5</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope></search><sort><creationdate>20140701</creationdate><title>Effects of laccase-catalyzed rutin polymer fraction on adipogenesis inhibition in 3T3-L1 adipocytes</title><author>Jeon, Ji Kyeong ; Lee, Jihye ; Imm, Jee-Young</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-816ecc333b8270b6c4029c0730e797b7340b4fb7de95e0fa8b824afc92f8b4293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>3T3-L1 cells</topic><topic>Anti-adipogenic activity</topic><topic>Binding</topic><topic>Biochemistry</topic><topic>Ethers</topic><topic>Fatty acids</topic><topic>Laccase</topic><topic>Monomers</topic><topic>Oligomers</topic><topic>Polymerization</topic><topic>Proteins</topic><topic>Rutin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jeon, Ji Kyeong</creatorcontrib><creatorcontrib>Lee, Jihye</creatorcontrib><creatorcontrib>Imm, Jee-Young</creatorcontrib><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jeon, Ji Kyeong</au><au>Lee, Jihye</au><au>Imm, Jee-Young</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of laccase-catalyzed rutin polymer fraction on adipogenesis inhibition in 3T3-L1 adipocytes</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2014-07-01</date><risdate>2014</risdate><volume>49</volume><issue>7</issue><spage>1189</spage><epage>1195</epage><pages>1189-1195</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>•Laccase-catalyzed oxidation of rutin created novel biopolymers.•Rutin polymer fraction (RPF, hexamer to octamer) effectively suppressed triglyceride accumulation compared to the rutin monomer.•The anti-adipogenic effect of RPF was confirmed in the expression of adipogenic transcriptional factors.
Rutin was enzymatically polymerized by laccase and the rutin polymer fraction (RPF) was collected by ultrafiltration with a molecular cut off of 3kDa. The RPF contained a range of oligomer size varying from hexamer (m/z 3650) to octamer (m/z 4866). The Fourier transform infrared spectrum indicated that various linkages such as hydrogen bonds (OH), aromatic groups (CC), and ether bonds (CO) were involved in rutin polymer formation. RPF more effectively suppressed triglyceride accumulation compared to the rutin monomer in 3T3-L1 adipocytes at all tested concentrations (p<0.05). The anti-adipogenic effect of RPF was mediated by down-regulation of the expression of adipogenic transcriptional factors including peroxisome proliferator-activated receptor (PPARγ), CCATT/enhancer binding protein (C/EBPα), sterol regulatory binding protein (SREBP1C) and fatty acid synthase (FAS) while up-regulating lipolysis and adiponectin expression in 3T3-L1 adipocytes. These results suggest that RPF has a potential protective effect in the development of obesity and that enzyme-catalyzed polymerization could be useful in improving flavonoids bioactivity.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2014.04.005</doi><tpages>7</tpages></addata></record> |
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| ispartof | Process biochemistry (1991), 2014-07, Vol.49 (7), p.1189-1195 |
| issn | 1359-5113 1873-3298 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | 3T3-L1 cells Anti-adipogenic activity Binding Biochemistry Ethers Fatty acids Laccase Monomers Oligomers Polymerization Proteins Rutin |
| title | Effects of laccase-catalyzed rutin polymer fraction on adipogenesis inhibition in 3T3-L1 adipocytes |
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