Effects of laccase-catalyzed rutin polymer fraction on adipogenesis inhibition in 3T3-L1 adipocytes

•Laccase-catalyzed oxidation of rutin created novel biopolymers.•Rutin polymer fraction (RPF, hexamer to octamer) effectively suppressed triglyceride accumulation compared to the rutin monomer.•The anti-adipogenic effect of RPF was confirmed in the expression of adipogenic transcriptional factors. Rutin was enzymatically polymerized by laccase and the rutin polymer fraction (RPF) was collected by ultrafiltration with a molecular cut off of 3kDa. The RPF contained a range of oligomer size varying from hexamer (m/z 3650) to octamer (m/z 4866). The Fourier transform infrared spectrum indicated that various linkages such as hydrogen bonds (OH), aromatic groups (CC), and ether bonds (CO) were involved in rutin polymer formation. RPF more effectively suppressed triglyceride accumulation compared to the rutin monomer in 3T3-L1 adipocytes at all tested concentrations (p