Visible light induced cell damage of Gram positive bacteria by N-doped TiO2 mesoporous thin films

Highly ordered N-doped titania mesoporous inorganic–organic hybrid thin films were prepared according to a refined recipe by an evaporative induced self-assembly route using Pluronic P123 as the template. Films were characterized by a grazing incident small angle X-ray scattering, atomic force microscopy, X-ray photoelectron spectroscopy, Raman spectroscopy and X-ray diffraction. Visible light induced bactericidal activity was performed on Gram-positive bacteria (Bacillus amyloliquifacience (NCIM 2829)). Bacterial cell death was confirmed by cell viability assay and inhibition of bacterial growth against reactive oxygen species induction is reported. ► N-doped mesoporous titania thin films were prepared by using sol–gel process. ► The titania thin films have highly ordered Im3m cubic crystalline arrangement. ► Due to N-doping the band gap of the titania thin films was reduced. ► The bactericidal activity under visible light irradiation was tested.