The effect of fullerenol C sub(60)(OH)~ sub(30) on the alcohol dehydrogenase activity irradiated with X-rays

In the present study the effect of X-irradiation on the alcohol dehydrogenase (ADH) activity in the presence of nanoparticles of fullerenol C sub(60)(OH)~ sub(30) under aerobic conditions was investigated in order to assess the potential radioprotective properties of fullerenol Fullerenol at 75 mg/mL decreased the radiation yield of inactivation of ADH irradiated with fullerenol by 20% comparing to ADH irradiated without fullerenol. Under conditions used during irradiation, 50% of *OH radicals could react with fullerenol and 50% could react with ADH Thus, it can be assumed that protective effect of fullerenol on the radiation inactivation of ADH was mostly due to scavenging *OH radicals by fullerenol. Moreover, fullerenol did not protect against post-irradiation damage as the G sub(inact) for ADH irradiated with fullerenol was still 20% lower than for ADH irradiated without fullerenol after 24 h from irradiation Additionally, fullerenol at 75 mg/L had no influence on the activity of unirradiated ADH up to 24 h We concluded that fullerenol C sub(60)o(OH)~ sub(30) protected ADH against radiation inactivation due to simple competition for the *OH radicals and did not modify its activity by association with the protein as it was proved in our previous papers for erythrocyte membrane proteins