Development of an in vitro model for the analysis of bovine endometrium using simple techniques

This study aimed to develop an in vitro model for the analysis of the bovine endometrium. Immunofluorescent staining revealed that the hetero‐spheroids and the cultured explants showed almost similar structure in the localization of bovine endometrial epithelial cells and endometrial stromal cells,...

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Veröffentlicht in:	Animal science journal 2015-05, Vol.86 (5), p.523-531
Hauptverfasser:	Yamauchi, Keisuke, Yamauchi, Nobuhiko, Yamagami, Kazuki, Nakamura, Nobuhisa, Yamashita, Seiya, Islam, Md. Rashedul, Tabata, Shoji, Yahiro, Kanji, Tamura, Tomoko, Hashizume, Kazuyoshi, Hattori, Masa‐aki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals bovine Cattle cell culture Cells Cells, Cultured endometrial explants endometrium Endometrium - cytology Endometrium - metabolism epithelial cells Estradiol - pharmacology Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism estrogen receptors Estrogens explants Female Fluorescent Antibody Technique gelatin gene expression hepatocyte growth factor Hepatocyte Growth Factor - genetics Hepatocyte Growth Factor - metabolism hetero-spheroid interferons Matrix Metalloproteinases - genetics Matrix Metalloproteinases - metabolism messenger RNA metalloproteinases Models, Biological oxytocin receptors progesterone receptors Real-Time Polymerase Chain Reaction Receptors, Interferon - genetics Receptors, Interferon - metabolism Receptors, Oxytocin - genetics Receptors, Oxytocin - metabolism Receptors, Progesterone - genetics Receptors, Progesterone - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism Spheroids, Cellular - metabolism Staining and Labeling stromal cells Zoology
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Zusammenfassung:	This study aimed to develop an in vitro model for the analysis of the bovine endometrium. Immunofluorescent staining revealed that the hetero‐spheroids and the cultured explants showed almost similar structure in the localization of bovine endometrial epithelial cells and endometrial stromal cells, except the glandular‐like structure of the epithelial cells inside the explants. Gelatin zymography revealed that the hetero‐spheroids did not express matrix metalloproteinases (MMPs) after 4 days of culture, but strong MMP expressions were observed in the cultured explants until 7 days of culture. Additionally, expression of progesterone receptor (PR), estrogen receptor alpha (ERα), type I interferon receptor 1 (IFNAR1) and 2 (IFNAR2) messenger RNA was observed both in the homo‐ and hetero‐spheroids. The expression of oxytocin receptor (OTR) mRNA in E2 and E2+P4 (1,3,5(10)‐Estratrien‐3, 17β‐diol + 4‐Pregnen‐3, 20‐dinone) treated groups were significantly (P
ISSN:	1344-3941 1740-0929
DOI:	10.1111/asj.12318