Detection of Mycoplasmas in Cultures of Myeloma Cell Lines and Hybridomas by Fluorescence Method Using Vero Cells as Indicators

Detection of Mycoplasmas in Cultures of Myeloma Cell Lines and Hybridomas by Fluorescence Method Using Vero Cells as Indicators

Four myeloma cell lines and four hybridomas were examined for the presence of mycoplasmas by the fluorescence method using bisbenzimide 33258 (Hoechst). The cell suspensions under examination were cultivated for 3 days together with Vero cells as indicators and then examined by fluorescence microsco...

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Veröffentlicht in:Acta veterinaria Brno 1991-01, Vol.60 (1), p.41-49
Hauptverfasser: Fischer, O., Granátová, Marta, Hájková, Marie, Nevoránková, Zora, Jurmanová, Květoslava
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container_end_page 49
container_issue 1
container_start_page 41
container_title Acta veterinaria Brno
container_volume 60
creator Fischer, O.
Granátová, Marta
Hájková, Marie
Nevoránková, Zora
Jurmanová, Květoslava
description Four myeloma cell lines and four hybridomas were examined for the presence of mycoplasmas by the fluorescence method using bisbenzimide 33258 (Hoechst). The cell suspensions under examination were cultivated for 3 days together with Vero cells as indicators and then examined by fluorescence microscopy. Mycoplasma contamination was detected in 2 myeloma lines. The results were confirmed by culture methods and by scanning electron microscopy.
doi_str_mv 10.2754/avb199160010041
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title Detection of Mycoplasmas in Cultures of Myeloma Cell Lines and Hybridomas by Fluorescence Method Using Vero Cells as Indicators
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