Detection of Mycoplasmas in Cultures of Myeloma Cell Lines and Hybridomas by Fluorescence Method Using Vero Cells as Indicators

Four myeloma cell lines and four hybridomas were examined for the presence of mycoplasmas by the fluorescence method using bisbenzimide 33258 (Hoechst). The cell suspensions under examination were cultivated for 3 days together with Vero cells as indicators and then examined by fluorescence microscopy. Mycoplasma contamination was detected in 2 myeloma lines. The results were confirmed by culture methods and by scanning electron microscopy.