Regulation of the expression of lamins A and C is post-transcriptional in P19 embryonal carcinoma cells

The polypeptide composition of the nuclear lamina can display important variations: undifferentiated cells express only lamin B and they acquire lamins A and C only after differentiation. We have analyzed the expression of lamins A and C in P19 pluripotent mouse embryonal carcinoma cells. Undifferen...

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Veröffentlicht in:	Biochemical and biophysical research communications 1992-12, Vol.189 (3), p.1639-1644
Hauptverfasser:	Lanoix, Jacqueline, Skup, Daniel, Collard, Jean-François, Raymond, Yves
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell Nucleus - physiology Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Neoplastic Kinetics Lamin Type A Lamin Type B Lamins Mice Molecular and cellular biology Molecular genetics Nuclear Proteins - genetics Poly A - genetics Poly A - isolation & purification RNA - genetics RNA - isolation & purification RNA Processing, Post-Transcriptional RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Neoplasm - genetics RNA, Neoplasm - isolation & purification Teratoma Time Factors Transcription, Genetic Tumor Cells, Cultured
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Zusammenfassung:	The polypeptide composition of the nuclear lamina can display important variations: undifferentiated cells express only lamin B and they acquire lamins A and C only after differentiation. We have analyzed the expression of lamins A and C in P19 pluripotent mouse embryonal carcinoma cells. Undifferentiated P19 cells are completely devoid of lamins A and C. We show that undifferentiated P19 cells contain low, but detectable steady-state levels of RNAs for lamins A and C that begin to increase by 24 h of retinoic acid-induced differentiation. However, the rate of transcription of the lamin A and C gene(s), analyzed by run-on transcription assays, remains unchanged during the differentiation process. These results demonstrate that, at least in P19 embryonal carcinoma cells, regulation of the expression of lamins A and C is a post-transcriptional event.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(92)90265-M