Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing

A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of K...

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Veröffentlicht in:	Journal of microbiological methods 2014-05, Vol.100, p.42-45
Hauptverfasser:	Monteiro, Jussimara, Widen, Raymond H., Pignatari, Antonio C.C., Kubasek, Carly, Silbert, Suzane
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - classification Bacterial Proteins - genetics beta-Lactamases - classification beta-Lactamases - genetics Genetic Variation Humans Klebsiella Infections - epidemiology Klebsiella Infections - microbiology Klebsiella pneumoniae Klebsiella pneumoniae - classification Klebsiella pneumoniae - enzymology Klebsiella pneumoniae - genetics Klebsiella pneumoniae carbapenemase KPC Molecular Epidemiology - methods Pyrosequencing Real-time PCR Sequence Analysis, DNA - methods
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Beschreibung
Zusammenfassung:	A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2014.02.013