Cloning, prokaryotic expression and homology modeling analysis of midgut aminopeptidase gene PxAPNS in Plutella xylostella (Lepidoptera: Plutellidae)

Cloning, prokaryotic expression and homology modeling analysis of midgut aminopeptidase gene PxAPNS in Plutella xylostella (Lepidoptera: Plutellidae)

The aim of this study is to analyze the cloning, expression and homology modeling of midgut aminopeptidase gene PxAPN5 in the diamondback moth, Plutella xylostella. A aminopeptidase (APN) gene was cloned from the P. xylostella midgut, and bioinformatics analysis of the gene was performed. The PxAPN5...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:K'un ch'ung hsueh pao 2014-01, Vol.57 (11), p.1272-1280
Hauptverfasser: XU, Lian, GAO, Huan-Juan, PAN, Zhi-Zhen, ZHU, Yu-Jing, CHEN, Qing-Xi, LIU, Bo
Format: Artikel
Sprache:chi
Schlagworte:
Lepidoptera
Plutella xylostella
Plutellidae
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The aim of this study is to analyze the cloning, expression and homology modeling of midgut aminopeptidase gene PxAPN5 in the diamondback moth, Plutella xylostella. A aminopeptidase (APN) gene was cloned from the P. xylostella midgut, and bioinformatics analysis of the gene was performed. The PxAPN5 protein with aminopeptidase activity was successfully cloned and expressed, and it could bind to Cry2Ab. Prediction of characteristics and functions of mutation sites in PxAPN5 was carried out by homology modeling. These results laid the foundation for the functional research of PxAPN.
ISSN:0454-6296