A New In Vivo Magnetic Resonance Imaging Method to Noninvasively Monitor and Quantify the Perfusion Capacity of Three-Dimensional Biomaterials Grown on the Chorioallantoic Membrane of Chick Embryos

Adequate vascularization in biomaterials is essential for tissue regeneration and repair. Current models do not allow easy analysis of vascularization of implants in vivo , leaving it a highly desirable goal. A tool that allows monitoring of perfusion capacity of such biomaterials noninvasively in a cheap, efficient, and reliable in vivo model would hence add great benefit to research in this field. We established, for the first time, an in vivo magnetic resonance imaging (MRI) method to quantify the perfusion capacity of a model biomaterial, DegraPol ® foam scaffold, placed on the embryonic avian chorioallantoic membrane (CAM) in ovo . Perfusion capacity was assessed through changes in the longitudinal relaxation rate before and after injection of a paramagnetic MRI contrast agent, Gd-DOTA (Dotarem ® ; Guerbet S.A.). Relaxation rate changes were compared in three different regions of the scaffold, that is, at the interface to the CAM, in the middle and on the surface of the scaffold ( p