Effect of four lichen acids isolated from Hypogymnia physodes on viability of rat thymocytes

Effect of four lichen acids isolated from Hypogymnia physodes on viability of rat thymocytes

Four lichen acids, physodalic acid (F1), physodic acid (F2), 3-hydroxyphysodic acid (F3), and isophysodic acid (F4), were isolated from Hypogymnia physodes methanol extract using preparative reversed-phase high performance liquid chromatography and their structures were determined by UV, MS, 1H NMR...

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Veröffentlicht in:Food and chemical toxicology 2013-01, Vol.51, p.160-164
Hauptverfasser: Pavlovic, Voja, Stojanovic, Igor, Jadranin, Milka, Vajs, Vlatka, Djordjević, Iris, Smelcerovic, Andrija, Stojanovic, Gordana
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Ascomycota - chemistry
Biological and medical sciences
Cell Proliferation - drug effects
Cell Survival - drug effects
Cells, Cultured
Cytotoxicity
Dibenzoxepins - chemistry
Dibenzoxepins - pharmacology
Dose-Response Relationship, Drug
Hypogimnia physodes
Hypogymnia physodes
Isolation
Lichen acids
Lichens - chemistry
Male
Medical sciences
Membrane Potential, Mitochondrial - drug effects
Mitochondrial membrane potential
Molecular Structure
Oxidative stress
Rats
Rats, Wistar
Reactive Oxygen Species - metabolism
Structure-Activity Relationship
Thymocytes - drug effects
Toxicology
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Zusammenfassung:Four lichen acids, physodalic acid (F1), physodic acid (F2), 3-hydroxyphysodic acid (F3), and isophysodic acid (F4), were isolated from Hypogymnia physodes methanol extract using preparative reversed-phase high performance liquid chromatography and their structures were determined by UV, MS, 1H NMR and 13C NMR. This is the first report on the isolation of F4 from H. physodes. Isolated rat thymocytes were cultivated with increasing F1–F4 concentrations (0.1, 1, 10μg/well) and proliferative activity, viability, ROS (reactive oxygen species) production and MMP (mitochondrial membrane potential) disturbances were evaluated. Obtained results show significantly decreased thymocytes proliferation was observed when cells were treated with F1 (1μg, p
ISSN:0278-6915
1873-6351
DOI:10.1016/j.fct.2012.04.043