Somatic chromosome differentiation in Cypripedium segawai Masamune and C. japonicum Thunberg

The chromosome complement of Cypripedium segawai at somatic metaphase carried numerous C-bands and various CMA-negative bands at the interstitial region and positive and deep bands of the Hoechst 33258 and quinacrine mustard (QM) double-staining at the positions where were of the C-bands and the CMA-negative bands. The chromosome complement of Cypripedium japonicum at somatic metaphase exhibited no C-band and a few CMA-positive, bright bands and dots in certain chromosomes and positive but faint bands of the Hoechst 33258 and QM double-staining at the distal region. Chromosome differentiation of C. segawai and C. japonicum could be different from each other. This paper is constributed from Laboratory of Plant Chromosome and Gene Stock, Faculty of Science, Hiroshima University (Contribution Number 27).