Highly Selective In Vivo Imaging of Endogenous/Exogenous Phosphate Ion over ATP and PPi

A chemodosimeter was designed to function as a highly selective phosphate ion (Pi) sensor, showing a 91‐fold ratiometric fluorescence enhancement. The probe successfully visualized exogenous and endogenous apyrase‐catalyzed Pi generation and was the first probe able to trace the generation and enrichment of Pi through hemichannel closure in Sf9 cells. Nothing but phosphate: A chemodosimeter was designed to function as a highly selective phosphate ion (Pi) sensor, showing a 91‐fold ratiometric fluorescence enhancement. The probe successfully visualized exogenous and endogenous apyrase‐catalyzed Pi generation and was the first probe able to trace the generation and enrichment of Pi through hemichannel closure in Sf9 cells.