Assignment of genes to pulse-field separated chromosomes of Schizophyllum commune

Chromosomal DNAs of the basidiomycete Schizophyllum commune were separated by Contour-Clamped Homogeneous Electric Field Electrophoresis (CHEF). The estimated sizes of the chromosomal DNAs ranged from 4·7 Megabase pairs (Mbp) to 1·6 Mbp, totalling 35·6 Mbp. Using sequences from 20 cloned genes we ob...

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Veröffentlicht in:	Mycological research 1994, Vol.98 (6), p.689-693
Hauptverfasser:	Ásgeirsdóttir, Sigrídur A., Schuren, Frank H.J., Wessels, Joseph G.H.
Format:	Artikel
Sprache:	eng
Schlagworte:	basidiomycetes Biological and medical sciences chromosome mapping contour-clamped homogeneous electric field electrophoresis cytogenetic analysis DNA Fundamental and applied biological sciences. Psychology Fungi genes genetic regulation genetic variation karyotyping measurement Plant cytology, morphology, systematics, chorology and evolution Schizophyllum commune Thallophyta
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Zusammenfassung:	Chromosomal DNAs of the basidiomycete Schizophyllum commune were separated by Contour-Clamped Homogeneous Electric Field Electrophoresis (CHEF). The estimated sizes of the chromosomal DNAs ranged from 4·7 Megabase pairs (Mbp) to 1·6 Mbp, totalling 35·6 Mbp. Using sequences from 20 cloned genes we obtained hybridization signals to at least 8 out of 11 chromosomal DNAs. The mating-type genes A and B were mapped to the largest and the smallest chromosomal DNA band, respectively. Developmentally regulated genes which are under the control of the mating-type genes, were localized to five different chromosomal DNA bands.
ISSN:	0953-7562 1469-8102
DOI:	10.1016/S0953-7562(09)80418-8