Combined use of NGF/BDNF/bFGF promotes proliferation and differentiation of neural stem cells in vitro

•NGF, BDNF and bFGF are important neurotrophic factors in NSCs induction.•Using of a single factor may limit the effect of NSCs differentiation.•bFGF was able to maintain and promote the proliferation of NSCs.•Combined use of NGF/BDNF/bFGF improved NSCs differentiating into neurons. Neurotrophic factors can promote the proliferation and differentiation of neural stem cells (NSCs). Here we report that the possibility of using bFGF in combination with BDNF and NGF to promote proliferation and differentiation of NSCs in vitro. C57BL/6 mouse NSCs were cultured, passaged and stained by immunofluorescence for nestin and GFP. According to different neurotrophic factors added to NSCs, seven experiment groups (NGF, BDNF, bFGF, bFGF+NGF, bFGF+BDNF, NGF+BDNF and NGF+BDNF+bFGF) and a blank control group were established. One week after induction and differentiation, results showed that there was significant difference in the percentage of NSCs differentiating into neurons among the experiment groups. The percentage in the multi-factor groups was significantly higher than that in the single-factor groups (p