Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases
The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening ‘typical’ as well as ‘atypical’ Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of ‘typical’ and ‘atypical’ EAEC p...
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| Veröffentlicht in: | Journal of microbiological methods 2015-03, Vol.110, p.45-48 |
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| container_title | Journal of microbiological methods |
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| creator | Rawool, Deepak B. Vergis, Jess Vijay, Deepthi Dhaka, Pankaj Negi, Mamta Kumar, Manesh Nair, Amruta Poharkar, Krupali V. Kurkure, Nitin V. Kumar, Ashok Malik, S.V.S. Barbuddhe, S.B. |
| description | The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening ‘typical’ as well as ‘atypical’ Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of ‘typical’ and ‘atypical’ EAEC pathotype. |
| doi_str_mv | 10.1016/j.mimet.2015.01.008 |
| format | Article |
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All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c392t-6faf5dc102032dc97c0f48a7eafc61d5070ea9d82690b3c84a89079a8d98566e3</citedby><cites>FETCH-LOGICAL-c392t-6faf5dc102032dc97c0f48a7eafc61d5070ea9d82690b3c84a89079a8d98566e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0167701215000172$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25598135$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rawool, Deepak B.</creatorcontrib><creatorcontrib>Vergis, Jess</creatorcontrib><creatorcontrib>Vijay, Deepthi</creatorcontrib><creatorcontrib>Dhaka, Pankaj</creatorcontrib><creatorcontrib>Negi, Mamta</creatorcontrib><creatorcontrib>Kumar, Manesh</creatorcontrib><creatorcontrib>Nair, Amruta</creatorcontrib><creatorcontrib>Poharkar, Krupali V.</creatorcontrib><creatorcontrib>Kurkure, Nitin V.</creatorcontrib><creatorcontrib>Kumar, Ashok</creatorcontrib><creatorcontrib>Malik, S.V.S.</creatorcontrib><creatorcontrib>Barbuddhe, S.B.</creatorcontrib><title>Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases</title><title>Journal of microbiological methods</title><addtitle>J Microbiol Methods</addtitle><description>The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening ‘typical’ as well as ‘atypical’ Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of ‘typical’ and ‘atypical’ EAEC pathotype.</description><subject>Animals</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhoea</subject><subject>EAEC</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli - ultrastructure</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - genetics</subject><subject>fimA</subject><subject>Fimbriae Proteins - genetics</subject><subject>Fimbriae, Bacterial - genetics</subject><subject>HEp-2</subject><subject>Humans</subject><subject>PCR</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Virulence - genetics</subject><issn>0167-7012</issn><issn>1872-8359</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc-KFDEYxBtR3HH1CQTJcT10m6T_JQcPyzC6woIieg5fJ196MnQnY9I94Gv5hGZ3dvconhLCr6pIVVG8ZbRilHUfDtXsZlwqTllbUVZRKp4VGyZ6Xoq6lc-LTab6sqeMXxSvUjrQDNaNeFlc8LaVgtXtpvizO8G0wuKCJ8ESIN-238kCccTF-ZFYNw_RwUTSOqzeLWREj-QqP1-_JzZEEuHoDAFvSMTJwTAhMbigfjTc-QVjgHGMOOaUE5Jd0nuMTu8dEB0ml4U6nDCiITaGmezXGfy9I3g352jjIMZ9wHzVkDC9Ll5YmBK-eTgvi5-fdj-2N-Xt189ftte3pa4lX8rOgm2NZpTTmhste01tI6BHsLpjpqU9RZBG8E7SodaiASFpL0EYKdquw_qyuDr7HmP4tWJa1OySxmkCj2FNKpdb15LyTvwH2jY9pbLhGa3PqI4hpYhWHWP-ZvytGFV3u6qDut9V3e2qKFN516x69xCwDjOaJ83jkBn4eAYwN3JyGFXSDr1G43K_izLB_TPgLxQqt6Q</recordid><startdate>201503</startdate><enddate>201503</enddate><creator>Rawool, Deepak B.</creator><creator>Vergis, Jess</creator><creator>Vijay, Deepthi</creator><creator>Dhaka, Pankaj</creator><creator>Negi, Mamta</creator><creator>Kumar, Manesh</creator><creator>Nair, Amruta</creator><creator>Poharkar, Krupali V.</creator><creator>Kurkure, Nitin V.</creator><creator>Kumar, Ashok</creator><creator>Malik, S.V.S.</creator><creator>Barbuddhe, S.B.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>201503</creationdate><title>Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases</title><author>Rawool, Deepak B. ; 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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Diarrhea - microbiology Diarrhoea EAEC Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - ultrastructure Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics fimA Fimbriae Proteins - genetics Fimbriae, Bacterial - genetics HEp-2 Humans PCR Polymerase Chain Reaction - methods Virulence - genetics |
| title | Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases |
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