Evaluation of a PCR targeting fimbrial subunit gene (fimA) for rapid and reliable detection of Enteroaggregative Escherichia coli recovered from human and animal diarrhoeal cases

The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening ‘typical’ as well as ‘atypical’ Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of ‘typical’ and ‘atypical’ EAEC p...

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Veröffentlicht in:	Journal of microbiological methods 2015-03, Vol.110, p.45-48
Hauptverfasser:	Rawool, Deepak B., Vergis, Jess, Vijay, Deepthi, Dhaka, Pankaj, Negi, Mamta, Kumar, Manesh, Nair, Amruta, Poharkar, Krupali V., Kurkure, Nitin V., Kumar, Ashok, Malik, S.V.S., Barbuddhe, S.B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Diarrhea - microbiology Diarrhoea EAEC Escherichia coli Escherichia coli - genetics Escherichia coli - isolation & purification Escherichia coli - ultrastructure Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics fimA Fimbriae Proteins - genetics Fimbriae, Bacterial - genetics HEp-2 Humans PCR Polymerase Chain Reaction - methods Virulence - genetics
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Zusammenfassung:	The present study describes the utility of a chromosomal associated fimbrial subunit gene (fimA) for screening ‘typical’ as well as ‘atypical’ Enteroaggregative Escherichia coli (EAEC) by PCR and its possible role as a promising molecular marker for rapid detection of ‘typical’ and ‘atypical’ EAEC pathotype.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2015.01.008