Immunogenecity D Efficacy of Locally Prepared Montanide-oil Based H5N2 AI Vaccine Containing Flagellin As Immune Enhancer

Objective of the present study was to investigate the efficacy of different vaccine preparations associating inactivated Avian influenza (H5N2) antigen, Montanide ISA 70 as an adjuvant and flagellin as an immune-enhancer. Their Immunoenhancing effect in chickens after single vaccination dose (0.5 mL) of the prepared vaccines was assessed. Measurement of cell mediated immunity by lymphocyte blastogenesis at 3rd, 7th, 10th, 14th, 21st and 28th was estimated also phagocytic activity was estimated 3rd, 7th, 10th, and 14th days post vaccination. While humoral immune response, based on Haemagglutinin inhibition test was traced 36 weeks post vaccination. The results revealed that vaccination with the inactivated Avian influenza (H5N2) vaccine in combination with Montanide oil ISA 70 provides robust immunity with longer duration and the addition of flagellin as immune-enhancer ensures a significant higher antibodies titer and better cellular immune response than those vaccines not combined with flagellin.