Expression of the Lactobacillus casei lactate dehydrogenase gene in Bacillus subtilis

A gene for allosteric lactate dehydrogenase (LDH) of Lactobacillus casei ATCC393 was transferred into Bacillus subtilis. The LDH was produced in a growth-associated type, and comprised up to 40% of the total cellular protein. The maximum specific activity in the transformant was 208 U/mg protein whi...

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Veröffentlicht in:	Biotechnology letters 1994-06, Vol.16 (6), p.561-564
Hauptverfasser:	Kim, S.F, Baek, S.J, Pack, M.Y
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis Biological and medical sciences Biotechnology Fundamental and applied biological sciences. Psychology gene expression gene transfer Genetic engineering Genetic technics genetic transformation lactate dehydrogenase Lactobacillus casei ldh gene Methods. Procedures. Technologies starter cultures structural genes Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Zusammenfassung:	A gene for allosteric lactate dehydrogenase (LDH) of Lactobacillus casei ATCC393 was transferred into Bacillus subtilis. The LDH was produced in a growth-associated type, and comprised up to 40% of the total cellular protein. The maximum specific activity in the transformant was 208 U/mg protein which was approximately 16 times higher than in L. casei or in the previously constructed Escherichia coli transformant.
ISSN:	0141-5492 1573-6776
DOI:	10.1007/BF00128600