The influence of dihydrodiol conformation on the metabolic activation of cyclopenta[a]phenanthrenes

The present study was undertaken in order to rationalise the apparent biological inactivity of 15,16-dihydro-6- methylcyclopenta[a]phenanthren-17-one (4) when other methyl isomers of 15,16-dihydrocyclopenta[ a]phenanthren-17- one e.g. the 11-methyl derivative (2), display appreciable tumorigenicity. In vitro metabolism of the 6-methyl-ketone- 17-one (4) demonstrated that its principal metabolite was the 3,4-dihydro-3,4-rmdiol (3,4-dihydroxy-6-methyl-3,4,15,16- tetrahydrocyclopenta[ a]phenanthren-17-one) (5) which, is the case of the active 11-methyl derivative, is the proximate genotoxin. Thus the inactivity of this 6-methyl-17-ketone cannot be ascribed to lack of formation of the 3,4-dihydro- 3,4-diol, the precursor of the 3,4-diol-l,2-epoxides (the ultimate mutagens in this series). However, the 6-methyl-3,4- dihydro-3,4-diol exists in a pseudo-diaxial rather than a pseudo-diequatorial conformation characteristic of the 3,4- dihydro-3,4-diols of the other members of the series. It is therefore suggested that a diequatorial conformation in the dihydrodiol is essential to the metabolic activation of the cyclopenta[ a]phenanthren-17-ones.