Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin time
Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and applications has increased the demand to thoroughly understand the interaction of NDs with blood and its effect on blood coagulation. Here, we report on the study of adsorption of human blood p...
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| Veröffentlicht in: | Diamond and related materials 2013-10, Vol.39, p.73-77 |
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| creator | Mona, J. Kuo, C.-J. Perevedentseva, E. Priezzhev, A.V. Cheng, C.-L. |
| description | Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and applications has increased the demand to thoroughly understand the interaction of NDs with blood and its effect on blood coagulation. Here, we report on the study of adsorption of human blood plasma on various sized carboxylated nanodiamonds (cNDs) using UV/visible spectroscopy and Fourier transform infrared spectroscopy (FTIR). The adsorption of human blood plasma on 5nm and 100nm sized cNDs is confirmed from UV/visible spectra. FTIR shows minor change in the shape of amide I absorption peak (1600–1700cm−1) indicating that the protein secondary structure remains unaffected for human blood plasma. The influence of cNDs on the blood coagulation has been estimated using Activated Partial Thromboplastin Time (APTT) test. The APTT test is one of the standard tests used to investigate the efficacy of the intrinsic pathways of coagulation. The APTT test results indicate that 5 and 100nm cNDs with various concentrations (10–500μg/ml) do not show delay in time when coagulation was initiated through the intrinsic pathway.
•Nanodiamond interacts with blood and its effect on blood coagulation is studied.•Adsorption of human blood plasma is confirmed from UV/visible spectra.•FTIR results indicating that the protein secondary structure remains unaffected for human blood plasma.•APTT test indicates that nanodiamonds do not show delay when coagulation was initiated. |
| doi_str_mv | 10.1016/j.diamond.2013.08.001 |
| format | Article |
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•Nanodiamond interacts with blood and its effect on blood coagulation is studied.•Adsorption of human blood plasma is confirmed from UV/visible spectra.•FTIR results indicating that the protein secondary structure remains unaffected for human blood plasma.•APTT test indicates that nanodiamonds do not show delay when coagulation was initiated.</description><identifier>ISSN: 0925-9635</identifier><identifier>EISSN: 1879-0062</identifier><identifier>DOI: 10.1016/j.diamond.2013.08.001</identifier><language>eng</language><publisher>Elsevier B.V</publisher><subject>Adsorption ; Blood coagulation ; Blood plasma ; Coagulation ; Fourier transforms ; Human ; Human blood plasma ; Infrared spectroscopy ; Nanodiamond ; Nanostructure</subject><ispartof>Diamond and related materials, 2013-10, Vol.39, p.73-77</ispartof><rights>2013 The Authors</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-23fc2ea185440b2b22eddfc3d6560988c29183002f5d9aeb0be9505e78e6c5fe3</citedby><cites>FETCH-LOGICAL-c422t-23fc2ea185440b2b22eddfc3d6560988c29183002f5d9aeb0be9505e78e6c5fe3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0925963513001519$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids></links><search><creatorcontrib>Mona, J.</creatorcontrib><creatorcontrib>Kuo, C.-J.</creatorcontrib><creatorcontrib>Perevedentseva, E.</creatorcontrib><creatorcontrib>Priezzhev, A.V.</creatorcontrib><creatorcontrib>Cheng, C.-L.</creatorcontrib><title>Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin time</title><title>Diamond and related materials</title><description>Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and applications has increased the demand to thoroughly understand the interaction of NDs with blood and its effect on blood coagulation. Here, we report on the study of adsorption of human blood plasma on various sized carboxylated nanodiamonds (cNDs) using UV/visible spectroscopy and Fourier transform infrared spectroscopy (FTIR). The adsorption of human blood plasma on 5nm and 100nm sized cNDs is confirmed from UV/visible spectra. FTIR shows minor change in the shape of amide I absorption peak (1600–1700cm−1) indicating that the protein secondary structure remains unaffected for human blood plasma. The influence of cNDs on the blood coagulation has been estimated using Activated Partial Thromboplastin Time (APTT) test. The APTT test is one of the standard tests used to investigate the efficacy of the intrinsic pathways of coagulation. The APTT test results indicate that 5 and 100nm cNDs with various concentrations (10–500μg/ml) do not show delay in time when coagulation was initiated through the intrinsic pathway.
•Nanodiamond interacts with blood and its effect on blood coagulation is studied.•Adsorption of human blood plasma is confirmed from UV/visible spectra.•FTIR results indicating that the protein secondary structure remains unaffected for human blood plasma.•APTT test indicates that nanodiamonds do not show delay when coagulation was initiated.</description><subject>Adsorption</subject><subject>Blood coagulation</subject><subject>Blood plasma</subject><subject>Coagulation</subject><subject>Fourier transforms</subject><subject>Human</subject><subject>Human blood plasma</subject><subject>Infrared spectroscopy</subject><subject>Nanodiamond</subject><subject>Nanostructure</subject><issn>0925-9635</issn><issn>1879-0062</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNqFkU1LxDAQhoMouH78BCFHL62TtOk2J5HFL1jwoueQJlPM0iZrkgr-e1t273sY5jDP-w7zDiF3DEoGrHnYldbpMXhbcmBVCW0JwM7IirVrWQA0_JysQHJRyKYSl-Qqpd0McFmzFdk92RTiPrvgaejp9zRqT7shBEv3g06jpvPAax-OK6iey-VEne-HCb3BBdAmu1-dcRbpmJ0eaP6OYezC4pGdp9mNeEMuej0kvD32a_L18vy5eSu2H6_vm6dtYWrOc8Gr3nDUrBV1DR3vOEdre1PZRjQg29ZwydoKgPfCSo0ddCgFCFy32BjRY3VN7g---xh-JkxZjS4ZHAbtMUxJsWbNRLWEcxqt10IIWUkxo-KAmhhSitirfXSjjn-KgVreoHbqmJFa3qCgVXPKs-7xoMP55F-HUSXjltysi2iyssGdcPgHLqSU_g</recordid><startdate>20131001</startdate><enddate>20131001</enddate><creator>Mona, J.</creator><creator>Kuo, C.-J.</creator><creator>Perevedentseva, E.</creator><creator>Priezzhev, A.V.</creator><creator>Cheng, C.-L.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SR</scope><scope>8BQ</scope><scope>8FD</scope><scope>JG9</scope></search><sort><creationdate>20131001</creationdate><title>Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin time</title><author>Mona, J. ; Kuo, C.-J. ; Perevedentseva, E. ; Priezzhev, A.V. ; Cheng, C.-L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-23fc2ea185440b2b22eddfc3d6560988c29183002f5d9aeb0be9505e78e6c5fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adsorption</topic><topic>Blood coagulation</topic><topic>Blood plasma</topic><topic>Coagulation</topic><topic>Fourier transforms</topic><topic>Human</topic><topic>Human blood plasma</topic><topic>Infrared spectroscopy</topic><topic>Nanodiamond</topic><topic>Nanostructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mona, J.</creatorcontrib><creatorcontrib>Kuo, C.-J.</creatorcontrib><creatorcontrib>Perevedentseva, E.</creatorcontrib><creatorcontrib>Priezzhev, A.V.</creatorcontrib><creatorcontrib>Cheng, C.-L.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>Engineered Materials Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Materials Research Database</collection><jtitle>Diamond and related materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mona, J.</au><au>Kuo, C.-J.</au><au>Perevedentseva, E.</au><au>Priezzhev, A.V.</au><au>Cheng, C.-L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin time</atitle><jtitle>Diamond and related materials</jtitle><date>2013-10-01</date><risdate>2013</risdate><volume>39</volume><spage>73</spage><epage>77</epage><pages>73-77</pages><issn>0925-9635</issn><eissn>1879-0062</eissn><abstract>Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and applications has increased the demand to thoroughly understand the interaction of NDs with blood and its effect on blood coagulation. Here, we report on the study of adsorption of human blood plasma on various sized carboxylated nanodiamonds (cNDs) using UV/visible spectroscopy and Fourier transform infrared spectroscopy (FTIR). The adsorption of human blood plasma on 5nm and 100nm sized cNDs is confirmed from UV/visible spectra. FTIR shows minor change in the shape of amide I absorption peak (1600–1700cm−1) indicating that the protein secondary structure remains unaffected for human blood plasma. The influence of cNDs on the blood coagulation has been estimated using Activated Partial Thromboplastin Time (APTT) test. The APTT test is one of the standard tests used to investigate the efficacy of the intrinsic pathways of coagulation. The APTT test results indicate that 5 and 100nm cNDs with various concentrations (10–500μg/ml) do not show delay in time when coagulation was initiated through the intrinsic pathway.
•Nanodiamond interacts with blood and its effect on blood coagulation is studied.•Adsorption of human blood plasma is confirmed from UV/visible spectra.•FTIR results indicating that the protein secondary structure remains unaffected for human blood plasma.•APTT test indicates that nanodiamonds do not show delay when coagulation was initiated.</abstract><pub>Elsevier B.V</pub><doi>10.1016/j.diamond.2013.08.001</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Adsorption Blood coagulation Blood plasma Coagulation Fourier transforms Human Human blood plasma Infrared spectroscopy Nanodiamond Nanostructure |
| title | Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin time |
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