Adsorption of human blood plasma on nanodiamond and its influence on activated partial thromboplastin time

Ever increasing use of engineered nanodiamond (ND) into the human blood for various biomedical studies and applications has increased the demand to thoroughly understand the interaction of NDs with blood and its effect on blood coagulation. Here, we report on the study of adsorption of human blood plasma on various sized carboxylated nanodiamonds (cNDs) using UV/visible spectroscopy and Fourier transform infrared spectroscopy (FTIR). The adsorption of human blood plasma on 5nm and 100nm sized cNDs is confirmed from UV/visible spectra. FTIR shows minor change in the shape of amide I absorption peak (1600–1700cm−1) indicating that the protein secondary structure remains unaffected for human blood plasma. The influence of cNDs on the blood coagulation has been estimated using Activated Partial Thromboplastin Time (APTT) test. The APTT test is one of the standard tests used to investigate the efficacy of the intrinsic pathways of coagulation. The APTT test results indicate that 5 and 100nm cNDs with various concentrations (10–500μg/ml) do not show delay in time when coagulation was initiated through the intrinsic pathway. •Nanodiamond interacts with blood and its effect on blood coagulation is studied.•Adsorption of human blood plasma is confirmed from UV/visible spectra.•FTIR results indicating that the protein secondary structure remains unaffected for human blood plasma.•APTT test indicates that nanodiamonds do not show delay when coagulation was initiated.