Determination of the tensile mechanical properties of the segmented mitral valve annulus

Abstract The mitral valve annulus is a complex and irregular component of the mitral valve apparatus, serving both a structural and sphincteric role. We have sought to determine the mechanical properties of the mitral valve annulus segmentally. Twenty porcine hearts were dissected to isolate the annulus. The annulus was segmented into four sections: anterior, posterior, and left and right commissural sections. Ten of these were tensile tested to failure as control samples. The remaining ten were digested in order to fully isolate the annulus from the myocardium, and subsequently tensile tested to failure. Histological samples of each segment were analysed to determine collagen/annular content. Whole segments of muscular annulus were tensile tested to failure; the stress and strain at failure and location of failure were determined in these larger specimens. Our results demonstrated that the anterior annulus is stiffer than the posterior segment by a factor of approximately 27 at a 2% strain level, and approximately 13 at a 6% strain. There is a trend in the results that identifies that the muscular annulus is stiffest at the right commissural segment, while the posterior segment tends to be the least stiff. The stiffness of the samples can be correlated with the area associated with the dense collagen annulus using histological analysis. Finally, the weakest section of the mitral valve annulus was identified as the intersection of the right commissural segment and the posterior segment.