Simultaneous analysis of nitrate, nitrite, acetate and 3-hydroxybutyrate by HPLC

A HPLC-method for a simultaneous analysis of nitrate, nitrite, acetate and 3-hydroxybutyrate in a wide range of concentrations was developed. The paired ion chromatography is based on the method by H. Papen et al. [1]. The ranges of detectable concentrations were for nitrate and nitrite 0.05–300 mg/l, for acetate 10–1000 mg/l and for 3-hydroxybutyrate 10–2000 mg/l. The substances were detected with a dual wave UV-detector. Nitrate and nitrite were detected at two different wave-lengths (210 and 240 nm). Acetate and 3-hydroxybutyrate were detected at 210 nm. The method was not influenced by large amounts of phosphate, ammonium sulfate, magnesium sulfate and trace elements.